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Cellular Reprogramming Inc
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StemCore Laboratories
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Cellular Reprogramming Inc
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Roslin Cell Therapies
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ReproCELL
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Lonza
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BEHRINGER International GmbH
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Image Search Results
Journal: Human Molecular Genetics
Article Title: Aberrant mitochondrial function in patient-derived neural cells from CDKL5 deficiency disorder and Rett syndrome
doi: 10.1093/hmg/ddz208
Figure Lengend Snippet: An increase in oxygen consumption rate (OCR) in CDD NPCs. Seahorse Cell MitoStress test was performed to measure OCR in CDKL5-mutant and wild-type-expressing isogenic NPCs clones (n = 4; 2 wild-type, 2 mutant) following a sequential addition of inhibitors of mitochondrial function: oligomycin, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), and a combination of antimycin A and rotenone; (A) OCR Seahorse assay profile plot, (B) basal respiration, maximal respiration, ATP production and spare respiratory capacity. Basal respiration was calculated after subtraction of non-mitochondrial respiration. ATP-linked respiration was calculated following the addition of oligomycin. Maximal respiration was measured following the addition of FCCP. Spare respiratory capacity was calculated based on the difference between the basal respiration and maximal respiration. (C) Metabolic potential in immortalized CDD isogenic mutant and wild-type fibroblasts. Asterisks represent significance comparing OCR of mutant to wild-type; n = three well replicates per condition in 96-well assay plates, P < 0.05.
Article Snippet:
Techniques: Mutagenesis, Expressing, Clone Assay